RNA Interference, Editing, and Modification: Methods and Protocols

Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA
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Rosenberg et al. Annu Rev Genet — J Cell Biol 1 — Biomol Ther 5 4 — Genome Biol 13 12 Curr Top Microbiol Immunol — Semin Cell Dev Biol 23 3 — Biochimie — Nat Rev Mol Cell Biol 17 2 — Science — Nat Biotechnol 30 3 — Nat Commun Nat Methods 9 6 — Nat Methods 10 2 — Mysore et al. RNAi has attracted considerable attention as a pest control tool, particularly in the agricultural space.

In […]. In this paper, Fernando and colleagues have reported some results that may potentially facilitate future […]. Gillet et al. Thairu et al. RNAi works well in beetles, in general, and why that is is not all that clear but Yoon et al.

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The authors […]. Hu […]. It is well established that the RNAi response of Coleoptera and Lepidoptera differ greatly but less is known about the basis for these differences.

RNAi - RNA interference

Skukla et al systematically investigated this question and report their insights in a recent paper […]. The variability of dsRNA-induced RNA interference is well documented with some insects very amenable to this method of regulating gene expression while are others are very much less so. The factors involved in this variation are known to some extent […].

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MicroRNA and dsRNA targeting chitin synthase a reveal a great potential for pest management of the hemipteran insect Nilaparvata lugens. A recent review by Darrington et al. Biomedical researchers, clinicians and even insect biologists. Following biolistic delivery of gold particles carrying the appropriate recombinant pTomCT vectors, leaf explants were placed on regeneration medium containing spectinomycin for the selection of transformants. Relationship between mRNA stability and length: an old question with a new twist.

Plant-mediated RNAi for aphid control could be a significant tool in the near future and Yu et al. Lin et al. Directly feeding B. Whitten et al.

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A corn variety expressing a Diabrotica virgifera virgifera-specific double-stranded RNA was recently registered by the U. Environmental Protection Agency EPA for the limited purposes of seed increase and breeding purposes for a period of two years on acreage not to […]. Das et al.

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This work was published recently in BMC Biotechnology. Finding convenient modes of delivery of gene silencing RNAs has been ongoing since the […]. Abdellatef et al.

Plant-expressed dsRNA […]. Khajuria et al have published a short paper in Insect Biochemistry and Molecular Biology describing their observations supporting the idea that Diabrotica vigifera exhibits parental RNAi following the oral delivery of dsRNA. Ulrich et al have recently published an analysis of iBeetle results aimed at identifying potential targets for RNAi-based pest control. Forgot password? Remember Me? Forgot Password?

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In RNA Interference, Editing, and Modification: Methods and Protocols, hands-on experimentalists describe in detail the protocols and assays they have. RNA Interference, Editing, and Modification: Methods and Protocols; find Sigma- Aldrich-Z MSDS, related peer-reviewed papers, technical documents.

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